PLCζ or PAWP: revisiting the putative mammalian sperm factor that triggers egg activation and embryogenesis
نویسندگان
چکیده
منابع مشابه
Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.
Mammalian oocyte activation is mediated by cytosolic calcium (Ca(2+)) oscillations initiated upon delivery of a putative 'sperm factor' by the fertilizing sperm. Previous studies suggest the identity of this sperm factor as the testis-specific phospholipase C-zeta (PLCζ). Recently, a post-acrosomal sheath WW domain-binding protein (PAWP) has been proposed as an alternative sperm factor candidat...
متن کاملIs PAWP the “real” sperm factor?
Mammalian embryo development is initiated by intracellular Ca2+ oscillations that result in oocyte activation following gamete membrane fusion. It is widely believed that oocyte Ca2+ oscillations are triggered by a sperm-specific protein, phospholipase C-zeta (PLCζ) that activates InsP3 production leading to repetitive Ca2+ release from intracellular stores. However, a recent report in the FASE...
متن کاملRe: Is PAWP the ‘real’ sperm factor?
Mammalian embryo development is init iated by intracel lular Ca2+ oscillations that result in oocyte activation following gamete membrane fusion. It is widely believed that oocyte Ca2+ oscillations are triggered by a sperm-specific protein, phospholipase C-zeta (PLCζ) that activates InsP3 production leading to repetitive Ca2+ release from intracellular stores. However, a recent report in the FA...
متن کاملSperm-specific post-acrosomal WW-domain binding protein (PAWP) does not cause Ca2+ release in mouse oocytes.
Mature mammalian oocytes undergo a prolonged series of cytoplasmic calcium (Ca(2+)) oscillations at fertilization that are the cause of oocyte activation. The Ca(2+) oscillations in mammalian oocytes are driven via inositol 1,4,5-trisphosphate (IP3) generation. Microinjection of the sperm-derived phospholipase C-zeta (PLCζ), which generates IP3, causes the same pattern of Ca(2+) oscillations as...
متن کاملEvaluation of PLCζ and PAWP Expression in Globozoospermic Individuals
OBJECTIVE Globozoospermia is a rare type of teratozoospermia with incidence of 0.1% among infertile individuals. Phospholipase C zeta (PLCζ) and postacrosomal sheath WW domain binding protein (PAWP) are the main candidates in sperm taking responsibility for oocyte activation during fertilization. Therefore, we aimed to evaluate the expression of these two genes at RNA and protein levels in glob...
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ژورنال
عنوان ژورنال: MHR: Basic science of reproductive medicine
سال: 2015
ISSN: 1460-2407,1360-9947
DOI: 10.1093/molehr/gav009